Genscript Taq Polymerase Protocol

Regulationofmir-196bbyMLLanditsoverexpressionbyMLLfusions …
According to the manufacturer’s protocol. Reverse transcription was performed using the TaqMan MicroRNA Reverse Transcription Kit (Ap Taq DNA polymerase (GenScript, Piscataway, NJ). PCR products were analyzedwithethidiumbromidebyelectrophoresisona2%agarosegel. … Access Document

Morphological And Molecular Identification Of Pathogenic …
protocol of plant genomic DNA kit (TIANGEN BIOTECH CO., LTD, contains 0.1 U Taq Polymerase/μl, 500 μM dNTP each, 20 mM GenScript Biotechnology Co., Ltd. (Nanjing, China) carried out PCR … Fetch Doc

Gene Synthesis Using DNAWorks
And additives Specific PCR protocols Analytical gel Isolate desired products Assembly Protocol blueheronbio.com) DNA 2.0 (http://www.dna20.com/) Gene Script Corporation (http://www.genscript mutation rate / 1000 nt / duplication (Cline et al., Nucleic Acids Res 24 (1996)) Taq polymerase = 0 … Read Content

Blueberry Latent Virus: An Amalgam Of The Partitiviridae And …
For the BlScV protocol, the tissue was homogenized in a borate buffer, pH 8.2, containing 2% (w/v) using Taq polymerase (Genscript). Amplification was performed usingprimersBBLVdetF(5 … Doc Viewer

Isolation And Characterization Of Nuclear-encoded …
The protocol described in Renshaw et al. (2009) to isolate and characterize microsatellites. 19 PCR buffer, 0.5 U Taq DNA polymerase (Genscript), 0.5 lM of each forward and reverse primer (IDT), 200 lM … Content Retrieval

Conservation Of Synteny Between Guppy And Xiphophorus Genomes
Units of Taq DNA-polymerase, were carried out in 10 L volumes in 200 L microcentrifuge Initial PCR was conducted using a protocol similar to that for … Retrieve Here

FastCloning: A Highly Simplified, Purification-free, Sequence …
Dent cloning kits, such as CloneEZ (GenScript USA Inc., uses regular Taq DNA polymerase to add a single 3’-A ciency according to the QuickChange protocol. Thus, … Access Content

Design And Optimization Of A Novel Reverse Transcription …
RNA sequence was prepared by GenScript (Piscataway, NJ, USA). The pGS-21a, Cla1⁄Nco1 plasmid contained a The use of Tfi polymerase, rather than Taq polymerase substantially improved the one-step protocol, … Read Here

Supporting Online Material For – Identified As XMRV -Infected
The engineered XMRV gene sequences were synthesized by GenScript (Piscataway, NJ) and manufacturer’s protocol. The extracted nucleic acid was immediately used for cDNA synthesis The Taq polymerase reagent from 5 RT-PCR kits, 2 lots of Invitrogen Platinum Taq, … Retrieve Doc

Population Genetic Structure Of Aphis Glycines
polymerase chain reaction [PCR] premix; Epicenter Technologies, Madison, WI), 4 pmol of each ßuores-cently labeled forward and unlabeled reverse primer, 1UofTaq Polymerase (Genscript, Piscataway, NJ), and1 lofDNAtemplate( 8ng/ l).ForlociAF85, AF 86, and AF I, we used the M13 tagging protocol of … View Document