Frequent Contaminant Of Taq Polymerase With Dna

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Organisms which are a frequent contaminant of cell cultures. The sensitivity is up to 10~100 copies of target DNA. Taq polymerase . 0.2 . 0.2 . 0.2 . H2O . 20.3 . 21.3 . 21.3 . A typical PCR program with Taq is shown below: … View Doc

Presence Of Murine Leukemia Virus (MLV)-related Virus Gene …
Frequent contamination of Taq polymerase with DNA. Clin Chem 1990;36:1258-9. mia viral genome contaminant in a commercial RT-PCR Kit is amplified using standard primers for XMRV. … Fetch Content

Molecular Survey Of Aeroplane Bacterial Contamination
UV light for 15 min to cross-link double-stranded DNA before the addition of Taq polymerase and template DNA. most frequent types of organisms present in the samples are laboratory contaminant is now considered an opportunistic … Fetch Document

DNA Profiling – Wikipedia, The Free Encyclopedia
Exponentially growing number of copies of the DNA of interest. The denaturation is generally performed by heating, and in this case using, replication enzymes that are tolerant of high temperatures (Taq DNA polymerase). However, DNA database searches showed much more frequent than expected false DNA … Read Article

Cat.# Part# 9PIM750
Taq DNA Polymerase Activity Assay: Taq DNA Polymerase activity is confirmed before the sTTP (a mix of unlabeled and [3H] dTTP); 10µg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50µl. Contaminant Avoid exposure to frequent temperature changes. Mix well prior to use. … Document Viewer

ORIGINAL PAPER – Incidence Of Fungal Contamination In A …
The most frequent genera in all production areas, identified by the colonies morphology and In the second approach, each Petri dish containing contaminant fungi from different primer at 0.20 mM, each dNTP at 0.10 mM, 10 μl 10x PCR buffer and 2.5 U Taq DNA polymerase in a final … Content Retrieval

PCR Analyses Of TRNA Intergenic Spacer, 16S-23S Internal …
Subject to frequent insertion-deletion events, such as rsl in The five contaminant strains of E. cloacae were isolated from three (Amersham-Pharmacia Biotech); 25 pmol of each primer;2UofTaq DNA polymerase (Gibco-BRL); and 5 l of DNA. … Return Document

DOI09848
Describe the ancient DNA contamination issues related to this statement (i.e_, can you be more specific about the contaminant's degrades into small fragments with frequent sites of molecular damage. Thus, although we can extract DNA consisting of PCR cocktail, Taq DNA polymerase, and 3 … Fetch Here

Virus PCR Assay Panels: An Alternative To The Mouse Antibody …
Most frequent contaminant, although reovirus, LCMV, minute virus of mice vitro amplification of a particular DNA sequence (e.g., a viral gene) in a sample to easily detectable levels, using a thermostable DNA polymerase enzyme (usually Taq poly- … Read Content

Part# 9PIM774 Revised 10/09 PCR Satisfaction Guarantee
Freeze-thaw cycles and exposure to frequent Contaminant Assays Endonuclease Assay: To test for endonuclease activity, 1µg of lambda DNA is incubated with 12.5 units of Pfu DNA Polymerase for 8 hours at 45°C followed by 8 hours at Pfu DNA Polymerase is lower than that of Taq DNA Polymerase. … Get Content Here

Fine Characterization Of The Iceman’s MtDNA Haplogroup
Lowed, including frequent treatment with bleach. units Taq polymerase (Ampli Taq Gold, Perkin Elmer, ature) to eliminate contaminant DNA. The DNAse was … Read More