Fermentas Taq Polymerase

Genetic Transformation Of Olive Somatic Embryos Through …
ACA TCG CTC AGA AGA ATC AG-3' and Fermentas Taq polymerase. Aliquots of each reaction were run on 1% agarose/ TBE 0.5× gel and visualized with ethidium bromide staining. i Determination of free proline content The proline … Get Content Here

T7 RNA polymerase – Wikipedia, The Free Encyclopedia
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA in the 5'→ 3' direction. T7 polymerase is extremely promoter -specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a DNA template and Mg 2+ ion as … Read Article

TM Last Updated: September 27, 2010 Product Information
2.5-10 units of a Hot Start Taq DNA polymerase 0.1-0.5 µM (final concentration) each of primer Template Set the cycling parameters as follows when Fermentas Hot Start Taq, Platinum Taq, AccuPrime Taq or Platinum Tfi is used: o95 C – 2 minutes 45 cycles of o95 C – 5 seconds … Read Here

Eppendorf Taq DNA polymerase with its unique Mg2+ self-adjusting buffer (Taq Buffer advanced) and five competitors’ kits with their reaction buffers. The PCR performance of M = Gene Ruler 100 bp DNA Ladder Plus (Fermentas … Access Content

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Hot Start Taq Fermentas 2.5-10 units of a HotStart Taq DNA polymerase 0.1-0.5 µM (final concentration) each of primers Template Di-water to a total volume of 20 µL/reaction. 2) Carry out the reaction and detection using one of the following … Doc Viewer

TA Cloning – Wikipedia, The Free Encyclopedia
PCR products are usually amplified using Taq DNA polymerase which preferentially adds an adenine to the 3' end of the product. Such PCR amplified inserts are cloned into linearized vectors that have complementary 3' thymine overhangs. … Read Article

Random Gene Dissection: A Tool For The Investigation Of …
PUC57 N.A. Ap Fermentas UAB pACYC184 N.A. Cm, Tc (1) pBR322 N.A. Ap, Tc (2) pBfiIRM14 bfiIR, bfiIMC1, bfiIMC2 Ap (3) pBRmTn10-dK-mNotI kan, tnp Ap, Km The plasmid was constructed in two steps. PCR-amplified using Taq DNA polymerase (Fermentas UAB), pBfiIRM14 DNA as a … Doc Retrieval

Epidemiologic And Virologic Investigation Of Hand, Foot, And …
DNTP, 2.5 U Taq DNA polymerase (Fermentas), and Taq polymerase buffer (Fermentas). PCR cycling conditions included an initial denaturation step at 94°C for 5 min, fol-lowed by 35 cycles of 94°C for 20 s, 55°C for 30 s, and 72°C for 1 min. … View Full Source

Hybrid Authentication In Upland Cotton Through RAPD Analysis …
Of Taq polymerase (Fermentas) and 2.5 µl of 25ng of template DNA. Amplification conditions were maintained at 94 ºC for about 2 minutes and thermal cycler was programmed for 45 cycles of 1 min at 94 ºC (denaturation), 35 ºC for 1 min (annealing) and 72 … Retrieve Content

Transgenic Cabbage ( Brassica Oleracea Var. Capitata …
Fermentas. Taq polymerase was purchased from GenTaq, Singapore. Hybond N+ membranes were procured from Amersham, UK. All the reagents and plant hormones were of molecular biology grade (Sigma, USA). Plant Transformation Tropical cabbage (B. oleracea var. capitata) … Visit Document

Box Stock Fermentas Freezer 211 – 25.07.2007 RESTRICTION …
Fermentas Freezer 211 – 25.07.2007 Box Stock RESTRICTION ENDONUCLEASES ER0051 BamH I 4000 units BamH I 1 1 EP0401 Taq DNA Polymerase (recombinant), 100 units (5u/µl) 15 1 K0192 High Fidelity PCR Enzyme Mix 500 units (5u/µl) 17 2 … Read Content

Detection Of Subgroup J Avian Leukosis Virus Gene By …
System contained 5 μl 10×PCR buffer (without Mg2+; Fermentas, 5726 Afr. J. Microbiol. Res. Figure 1. Determination of the optimal conditions for the whole blood PCR. (A) The whole standard Taq DNA polymerase was added from lane 1 to 8 in 50 μl of PCR reaction with 1 μl positive … Get Doc