Fermentas Dreamtaq Polymerase

The Antiepileptic Drugs Phenobarbital And Carbamazepine …
Mixed dNTPs, and 1.5 U DreamTaq DNA polymerase (Fermentas). Specific primers for rOat3, rOatp1a5, rPCFT, rMrp1, rMdr1, and rBcrp were designed using PrimerSelect software (DNAStar Inc., Madison, WI). All gene-specific primer pairs and annealing temper- … Access Full Source

IDENTIFICATION OF MYOSTATIN GENE C.960delG LOCUS POLYMORPHISM …
And 0.5 units of DreamTaq™ DNA Polymerase and 1x buffer (Fermentas) in total solution 25 µl. Amplification in vitro within GeneAmp® PCR System 9700 (Applied Biosystems™) was done with the condition of pra-denaturation at 94 ºC for … Get Doc

Comparative Expression Analysis Of -like Genes Of …
In a 20 ml reaction mix containing 1.25 U DreamTaq DNA polymerase (Fermentas), 16S rRNA primers (0.2 mM each), RNA template (100 ng), MgCl 2 (final concentration 2.0 mM) and dNTPs (final concentration 0.2 mM). PCR products were electrophoresed in … Fetch Here

Mining And Characterization Of Sequence Tagged …
DreamTaq DNA Polymerase (Fermentas). PCR amplification was conducted on Applied Biosystems Veriti Thermal Cycler (Applied Biosystems, www.appliedbiosystems.com). The cycling conditions consisted of a touchdown regime as follows: an initial denaturing step … Fetch Full Source

Variants Analysis Of Human Mitochondrial Genome Mutation …
Polymerase DreamTaq enzyme (Fermentas). PCR process is done by Automatic engine thermal (Perkin Elmer) by 30 cycles. The early steps of the PCR process is the initial step denaturation at 95°C for 3 min, then go to program PCR cycles with each cycle … Fetch Here

DESMODESMUS COMMUNIS (CHLOROPHYTA) FROM ROMANIAN FRESHWATERS …
Of DreamTaq® DNA Polymerase (Fermentas, Burlington, Ontario, Canada) in the manufacture’s buffer, 1.5 mmol MgCl 2, 0.2 mmol dNTP, 0.4 µmol primer in a final volume of 50 µL. The tubes were . Annals of … Access Content

Efficient Extraction Of Total DNA From Mangrove Species …
In this project, DreamTaq. TM. DNA polymerase (Fermentas, Madison), highly active . Taq. polymerase, was used. It was also one of large factor creating the difficulty in fragment analysis. Since it can amplify easier than normal . Taq. … Access Content

Transovarial Transmission Of Rickettsia And Organ-specific …
130 primer, 10 mM dNTPs, 1X DreamTaq buffer an d 1 U DreamTaq DNA Polymerase (Fermentas). 131 PCR-amplified products were visualized on a 1% agarose gel containing ethidium bromide. To 132 detect Rickettsia in specific organs using PCR, B. tabaci midguts, stylets, primary salivary … View Full Source

AEM Accepts, Published Online Ahead Of Print On 6 July 2012 …
159 50 L): 1.5 U Taq DNA polymerase (DreamTaq; Fermentas Life Science, Vilnius, 160 Lithuania), Taq buffer containing a final magnesium concentration of 2.5 mM, dNTPs 161 (20 nmol each), 12.5 g bovine serum albumin and 25 pmol of each primer, were carried … Get Doc

One-stop polymerase Chain Reaction (PCR): An Improved PCR …
Kits ( Taq DNA polymerase, DreamTaq DNA polymerase, 2× Blue master mix) were purchased from Shenergy (Shanghai, China), Fermentas Fermentas (Lithuania) and DBI (Shanghai, China), respectively. (B) One-stop PCR performs well under … Visit Document