Extension Rate Taq Polymerase

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Accuracy Of Replication In The polymerase Chain Reaction …
Been obtained with regard to extension of a mismatched primer template (7). It is not known if T. aquaticus also contains a sepa-rate 3' to 5' exonuclease activity that may be associated with the polymerase in vivo. Other conditions known to reduce the fidelity of Taq polymerase in vivo are high MgCl2 in … Read Here

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Evaluation Of TITANIUM Taq DNA Polymerase For High-Throughput …
Spectrum obtained by using TITANIUM Taq DNA Polymerase in a nine-plex extension reaction (nine SNPs located on chromosome 6). of SNPs with a pass rate lower than 80%, our quality threshold. TITANIUM Taq DNA Polymerase had the highest overall … Visit Document

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AccuPrime Taq : A Next Generation Ling Huang DNA Polymerase
With AccuPrime™ Taq, extension does not occur at non-specific sites 1B. lower than the mutation rate of the high-est fidelity enzyme, a replicative enzyme of E. coli(7). Taq DNA polymerase … View Doc

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The Polymerase Chain Reaction
To template, that extends to a variable distance from the primer binding site, using the available separate deoxynucleotides in the reaction dATP, dCTP, dTTP, dGTP taq DNA polymerase has a higher error rate (no proof-reading 3' to 5' exonuclease activity) Cyclic reaction Post-extension involves … Fetch This Document

Хэрэглэгч:Bilguun.alt/Ноорог …
Can be utilized for PCR-mediated DNA mutagenesis, as higher Mn 2+ concentration increases the error rate Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75-80°C, and commonly a temperature of 72°C is … Read Article

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Product Codes KAPA Taq HotStart
Subunit, wild-type Taq DNA polymerase of the thermophilic The enzyme has an error rate of approximately 1 error per For AT rich templates and amplicons, extension may be performed at 68°C. … Retrieve Doc

Factors For Successful PCR – Polymerase Chain Reaction – PCR …
The traditional Taq polymerase has been replaced in many labs by higher fidelity enzymes (those that make Increased yield can be achieved by increasing the extension time about every 20 cycles, to Factors that Affect the Chemical Reaction Rate – Reaction Kinetics … Read Article

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PfuTurbo DNA Polymerase
Proofreading PCR enzymes.1 Like Pfu DNA polymerase, PfuTurbo DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and lower temperatures should result in fewer mispaired primer-extension reactions than occur with Taq DNA polymerase during the lower … Retrieve Here

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Platinum Taq DNA Polymerase
Taq Polymerase in a 30 cycle reaction (94°C for 1', 55°C for 1.5 to 2', 72°C for 1.5 to 3') retains 100% activity as defined by RATE Very processive enzyme. It has an extension rate of 35-100 nt/sec at 75°C [Wittwer (1991) BioTechniques 10(1), 76. … Get Content Here

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HotStarTaq PlusPCR Handbook
Extension rate: 2–4 kb/min at 72°C Half-life: 10 min at 97°C; 60 min at 94°C PCR products using QIAGEN Taq DNA Polymerase and ProofStart® DNA Polymerase or HotStar HiFidelity … Visit Document

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PCRBIO HS Taq DNA Polymerase
PCRBIO HS Taq DNA Polymerase has an error rate of approximately 1 error per 2.0 x 105 nucleotides Extension: Optimal extension is achieved at 72°C. The optimal extension time is dependent on amplicon … Visit Document

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DNA Polymerase Kit
Our Taq DNA Polymerase is a 94kDa thermostable recombinant DNA Polymerase derived fromThermus aquaticus. It can amplify fragments up to 5kb. Concentration: 5 units/ l Substrate analogs: dNTP, ddNTP, fluorescent dNTP/ddNTP Extension rate: 0.9~1.2 kb/min at 72°C Taq DNA Polymerase Kit Kit Contents : Taq DNA … Access Content