Endonuclease Activity Taq Polymerase

Taq Red DNA Polymerase Kit Components
One unit is defined as the amount of polymerase that incorporates nmoles of dNTPs into acid-precipitable DNA in 30 minutes at 72 °C under standard assay conditions. Quality Control Taq Red DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking … Retrieve Document

RTaq (recombinant Taq) DNA Polymerase
RTaq (recombinant Taq) DNA Polymerase Code No. TAP-Lot No. ***** Storage Store at -20℃ at 75℃, no endonuclease activity was observed after agarose gel electrophoresis. … View This Document

Enzyme Biotechnology – Everyday Uses For Enzymes – Enzymes …
With time, research, and improved protein engineering methods, many enzymes have been genetically modified to be more effective at the desired temperatures, pH, or under other manufacturing conditions typically inhibitory to enzyme activity (eg. harsh chemicals), making them more suitable and … Read Article

Klenow Fragment – Wikipedia, The Free Encyclopedia
Because the 5' → 3' exonuclease activity of DNA polymerase I from E. coli makes it unsuitable for many chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase. Replication factor C · Flap endonuclease · Topoisomerase · Replication protein A … Read Article

Diamond Taq
Coli bacterium containing the Thermus aquaticus DNA Polymerase gene. This thermophilic eubacterium strain lacks Taq I restriction endonuclease. The expressed enzyme shows very good fidelity and catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. … Retrieve Here

Tfi DNA Polymerase
Tfi DNA Polymerase is functionally tested for amplification and the absence of double- and single-stranded endonuclease activity, as well as the Tfi DNA Polymerase can be used in protocols that currently use Taq DNA Polymerase without modification. … Retrieve Document

DNA polymerase I – Wikipedia, The Free Encyclopedia
Its role in replication was proven when, in 1969, John Cairns isolated a viable Polymerase I mutant that lacked the polymerase activity. Cairns' lab assistant Paula De Lucia Replication factor C · Flap endonuclease · Topoisomerase · Replication protein A . Eukaryotic DNA polymerase: delta (POLD1 … Read Article

GENET BIO
Quality Control: Endonuclease, Exonuclease, DNase, RNase and Protease activity is not detected. Prime TaqTM DNA Polymerase is determined to be >90% pure as judged by SDS-PAGE. … Get Document

Super-Run Taq DNA Polymerase
Nicking activity, endonuclease and exonuclease activity were not detected after the incubation of 1 tubes of 5 μl Super-Run Taq DNA polymerase 1 tube of 100 ul 10X Super-Run Taq Buffer (20 mM Mg … Document Viewer

RTaq (recombinant Taq) DNA Polymerase
RTaq (recombinant Taq) DNA Polymerase Code No. TAP-201 Lot No. ***** Storage Store at -20℃ at 75℃, no endonuclease activity was observed after agarose gel electrophoresis. … View Full Source

Polymerase – Wikipedia, The Free Encyclopedia
One particular polymerase, from the thermophilic bacterium, Thermus aquaticus (Taq) (PDB, EC 2.7.7.7) is of vital commercial importance due to its use in the polymerase chain Replication factor C · Flap endonuclease · Topoisomerase · Replication protein A . Eukaryotic DNA polymerase: … Read Article

Basic Principles Of Real-time Quantitative PCR
Extension phase, the 5´ endonuclease activity of the Taq DNA polymerase cleaves the probe, which separates reporter and quencher dyes and fluorescence is detected … Fetch Content

OptimizationofQuantitative MultiplexRT-PCR
Of the 5' →3' endonuclease activity of Taq DNA polymerase to cleave fluorescently labeled oligonucleotide probes that have hybridized with … Fetch Here

Product Information Purity (SDS‐PAGE) Taq‐B DNA Polymerase
Activity (est.) 74,625 U/mg SS Exonuclease 50 U <5.0% released DS Exonuclease 50 U <0.5% released Endonuclease 50 U <10% converted E.coli 16S rDNA Contamination 50 U <10 copies Storage -20°C Product Information Taq‐B DNA Polymerase Taq Polymerase: … Visit Document