Difference Between Pfu Taq Polymerase

PCR Development
The main difference between Pfu and alternative enzymes is Pfu's superior thermostability and proofreading properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity, meaning that it work its way along the … Access Document

Easy-A High-Fidelity PCR Cloning Enzyme And Master Mix
• Cloning efficiencies comparable to Taq DNA polymeraseDNA polymerase • Fidelity equivalent to Pfu DNA polymeraseDNA polymerase Cloning effi ciencies are equivalent to those obtained with Taq DNA polymerase (even for a 4.0 kb fragment) with no difference in colony number. … View Full Source

Bio572: Additional Cloning Methods
As you recall from the last lecture, much was made of the difference between accepting PCR products made with Taq polymerase, an enzyme that has a with dATP and a 3'-5' exonuclease (Pfu polymerase, T4 DNA polymerase, Klenow, … Document Retrieval

BRIEF REPORTS Cloning And Sequence Analysis Of Gene OipA …
3.0 U Pfu-Taq polymerase, 100 ng DNA template and 1×PCR buffer (pH8.8). The parameters for PCR were at 94 for 4 min, just as the difference between HP0638 and JHP0581. The other is that variation of the same strain induced the difference. … Retrieve Document

4. Modern Techologies
V. Extension or Polymerisation: •Taq polymerase incorporates about 2000 nucleotides/minute at optimal Pfu – (Pyrococcus furiosus) Natural Pwo – (Pyrococcus wosei) Natural WHAT IS THE DIFFERENCE BETWEEN PCR AND REALTIME PCR ? … Read Content

Ll|llll Manual Supplement
The second difference is the presence of dideoxynucleotide triphosphates in the sequencing reactions cient DNA polymerase from Pyrococcus furiosis or Taq DNA polymerase and [3SS]dATP. Pfu polymerase does not … View Doc

Product CatalogProduct Catalog
Primary difference between polymerases in Atlas and Titan product-lines is the strain of E. coli used for Atlas Pfu DNA Polymerase 2500U BA00505 Titan Taq DNA Polymerase 500U BT10101 … Access Document

Synergy between DNA polymerases Increases polymerase Chain …
Reaction,amixtureofrecombinant Taq andcloned Pfu polymerase, significant difference between the alternative DNA polymerase– [11] M.B. Kermekchiev, L.I. Kirilova, E.E. Vail, W.M. Barnes, Mutants of Taq DNA polymerase … Retrieve Content

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
The main difference between Pfu and alternative enzymes is Pfu's superior thermostability and 'proofreading' properties compared to Taq DNA polymerase. … Read Article

Variants Of PCR – Wikipedia, The Free Encyclopedia
Faststart polymerase is a variant of Taq polymerase that requires strong heat activation, thereby avoiding non-specific amplification due to polymerase activity at low temperature (see hot-start PCR above). … Read Article

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase ( / ˌ t æ k ˈ p ɒ l ɨ m ər eɪ z /) is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was Some thermostable DNA polymerases have been isolated from other thermophilic bacteria and archaea, such as Pfu DNA polymerase, possessing a proofreading … Read Article

Significant difference between the GEU produced by re- restriction digestion to excise out the fragment, one could blend of high processive Taq DNA polymerase and a sec- ond Pfu DNA polymerase (error rate: I .6 x 10-6 errors/bp)l … Doc Retrieval

AccuPrime Pfx : High-fidelity DNA Kyusung Park
Than that of Taq and two times that of Pfu DNA polymerase (1). PCR Optimization for AccuPrime™ Pfx DNA Polymerase.Though the perform-ance of AccuPrime™ Pfx DNA polymerase … Fetch Here