Boehringer Mannheim Taq Polymerase

A Molluscan Peptide -amidating Enzyme Precursor That …
Of cDNA ends; RT-PCR, reverse transcriptase polymerase chain reaction; TbH, Drosophila tyramine b-hydroxylase; TGN, trans-Golgi network; TMT, TM with 1% Triton X-100. Taq polymerase (Boehringer Mannheim, Germany), by using 50 pmol PAM-speciļ¬c antisense primer in combination with 50 pmol RoRi-(dT 17 … Read Content

Application Of PCR Assay To Differentiate Two Subtypes Of …
Reverse primers for H1 and H3, respectively, and Taq polymerase ( Boehringer Mannheim) in the following reaction conditions: 94 °C for 1 min followed by 35 cycles of 94 °C for 30 s, 46 °C for 30 s and 72 °C for 45 s. PCR products were analyzed by agarose gel … Content Retrieval

Still Glowing After All These Years: Storage Life Of A …
Ringer Mannheim, Basel, Switzerland). Nu- (Boehringer Mannheim, Basel, Swit-zerland), two sets of primers USA)), and Taq polymerase (Taq p) (2 IU) (AmpliTaq DNA Polymerase, Stoeffel Fragment, Perkin Elmer, Branch-burg, NJ, USA). The concentrations in the complete reaction mix were twice the … Access Content

An Important Role For FlhDC In The Control Of NucA Expression …
Restriction and modification enzymes were purchased from Boehringer Mannheim. Taq polymerase and PCR related products were from Perkin Elmer or Takara Biomedicals. Other chemicals were purchased from SIGMA. DNA manipulation and analysis DNA was … Retrieve Full Source

Expression Of A Cholesterol Oxidase Gene From Arthrobacter …
Galactopyranoside) were purchased from Boehringer Mannheim (Mannheim, (Taq polymerase), polymerase chain reaction (PCR) buffer and deoxynucleotides were purchased from Perkin-Elmer Cetus (Foster City, CA, USA). Oligonucleotides were synthe- … Get Content Here

Kary Mullis – Wikipedia, The Free Encyclopedia
The Taq polymerase was heat resistant and would only need to be added once, Preis Biochemische Analytik of the German Society of Clinical Chemistry and Boehringer Mannheim; 1991 – National Biotechnology Award | Gairdner Award | R&D Scientist of the Year; … Read Article

Hoffmann-La Roche – Wikipedia, The Free Encyclopedia
Puerto Rico in the US; Welwyn Garden City and Burgess Hill in the UK; Clarecastle in Ireland; Mannheim and Penzberg in Germany; Mississauga and Laval in Canada; Shanghai in China; Mumbai It bought the patents for the polymerase chain reaction technique in 1992. It manufactures and sells … Read Article

99-136-Oliver-U Of TE
Of dTTP and 50 µM digoxigenin-11’-dUTP (Boehringer Mannheim, Indianapolis, ID), 5 U of Taq polymerase (Promega, San Diego, CA), 5 µl 10X magnesium-free thermophilic buffer [500 m M KCl, 100 m M Tris-HCl (pH 9.0), and 1% Triton X-100], 4 µl of 25 m M … Fetch Full Source

Screening Of Blood Donors For HCV RNA By RT-PCR
Inhibitor, Taq polymerase- Boehringer Mannheim, Maloney Murine reverse transcriptase- Boehringer Mannheim) were used and amplified as follows: 37°C 45 min 94°C 3 min 94°C 1 min 60°C 1 min 72°C 1 min, 30 cycles 4°C soak After round a, PCR b reagent was added (10 X … Read Here

Polymerase Chain Reaction Is A Good Diagnostic Tool For …
Deoxynucleoside triphosphates (dNTP), 2.5 U Taq polymerase (Boehringer Mannheim, Germany) and 0.5 µM (each) of the primers. The primer sets used to amplify the 123-bp IS 6110 gene fragment consisted of TBC1 (CCT GCG AGC GTA GGC GTC GG) and TBC2 … Fetch Here

Characterization Of An Axonemal Dynein Heavy Chain Expressed …
L using Taq polymerase (Boehringer Mannheim, Indianapolis, IN) and Vent polymerase (New En-gland Biolabs, Beverly, MA) according to Barnes (17). One am-plified product, 1.13 kb in length, was obtained, cloned into the … Access This Document

Rapid Clinical Viruses From Detection Samples Transcriptase …
Taq polymerase amplification was performed onthe result-ingcDNAwiththeupstreamdenguevirus consensusprimer (D1). Lumi-Phos (Boehringer Mannheim) in accordance with the manufacturer's protocol. Visualization ofboundprobeswas … Fetch Content