Bioline Taq Polymerase Buffer

Eyespot Of Cereals Revisited: ITS Phylogeny Reveals New …
Gel in 0.5 ×TAE buffer (0.4M Tris, 0.05M NaAc and 0.01M EDTA, pH 7.85) and visualized under UV light. 843 Table 1. (Bioline) Taq polymerase. The ampliļ¬cation reactions were done on a GeneAmp PCR System 9600 (Perkin-Elmer, Norwalk,Connecticut). … Read Here

Blackwell Publishing LtdExtensive Polymorphism And …
Stored at –20 °C in 0.1 mL of TE buffer. Microsatellite genotyping We amplified nine microsatellites that were isolated originally in the peregrine falcon Falco peregrinus (Fp5, 0.2 U of Taq polymerase (Bioline), 1× PCR manufacturer-supplied buffer, 1.5 m m MgCl 2 … Read Full Source

Phylogenetics Of The Grass Genus Ehrharta: Evidence For …
50-jl PCR reaction contained 5 RA of lOX Taq polymerase buffer (Bioline, London, U.K.), 2.5 AL of 50 mM MgCl2, 4 [L of dNTP (1.25 mM), 2 pI of each primer (10 ItM), 0.5 pI of Taq polymerase (Bioline), 4 [L of template DNA (un- diluted or 1/10 diluted), and … Access Document

Extraction Of Bulk DNA From Thar Desert Soils For …
buffer, 1.5 mM MgCl2 and 2.5 units of Taq DNA polymerase (Bioline GmbH, Germany) in a 100 µl reaction volume. and 2.5 units of Taq DNA polymerase (Bioline GmbH, Germany) in a 100 µl reaction volume. Initial denaturation … Doc Retrieval

Restriction Fragment Length Polymorphism Analysis (RFLP) Of …
Taq DNA polymerase, MgCl 2 solution and Taq buffer were from Biotaq TMDNA Polymerase Bioline. Deoxyribonucleotides (dNTPs) were obtained from Boehringer Mannheim GmbH, Germany, at a concentration of 100 mM. Working stock solution of dNTPs was made … Visit Document

Introducing The MyTaq DNA Polymerase Family [HD] – YouTube
The MyTaq DNA Polymerase product range is a new generation of very high performance PCR products developed by Bioline, sensitivity and speed. The enzyme is supplied with an industry-leading novel buffer system, 0:49 Watch Later Error Taq Polymerase by demonstrativesinc 487 … View Video

Contribution Of Conserved ATP-Dependent Proteases Of …
PCRs were performed using Bioline Taq DNA polymerase and buffer supplied by DNA Technology. Construction of C. jejuni mutants. The plasmids used are listed in Table 1. C. jejuni NCTC11168 chromosomal DNA was used as a template for ampliļ¬cation. … Retrieve Document

TEF Mycosphaerella Spp. And Their Anamorphs
5x Colorless GoTaq Flexi buffer (Promega) 5,0 1x MgCl 2 (50 mM, Bioline) 2,0 2 mM dNTP's (10 mM each) 0,15 0,06 mM each EF1-728F (10 µM) 0,5 0,2 µM EF-2 (10 µM) 0,5 0,2 µM Taq DNA polymerase (5 U/µl, Bioline) 0,12 0,6 Unit subtotal 23,0 template 2,0 total 25,0. Title: Protocols Fungi … Document Retrieval

Bird Schistosomes Of Wildfowl In The Czech Republic And Poland
Taq polymerase buffer (Bioline), 2 mM MgCl2 (Bioline), 200 µM dNTP (Bioline) and 0.65 unit of Taq polymerase (Bio-line). The reaction temperature profile included the initial denaturation step at 95°C for 60 sec followed by 30 cycles of … Access Content

BIORON Contact Germany Phone: +49-(0)-621- 5720 915 Contact …
Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' 10xreaction buffer: 100mM ammonia sulfate 200mM TrisHCl, pH 8,8 100mM KCl 20mM MgSO 4 1% Triton X-100 1mg/ml BSA Reaction mixture: dNTPs mixture (8mM of each) – 0.5 µl … Read More

Chapter 2
SureClean (Bioline, Luckenwalde, Germany). 6. LB broth supplemented with 10 mM MgSO 4. 7. 7 mL Taq DNA polymerase buffer (10×), 6 mL 25 mM MgCl 2, 1 mL 100 mM dATP, and 1 mL Taq DNA polymerase (5 U/mL). Add H 2 O to a final volume of 70 mL. 2. Incubate at 72°C for 30 min. 3. … Retrieve Content