Axygen Taq Polymerase

Reports
Each primer, 1 U Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, Tubes (MCT-150-L-C; Axygen, Union City, CA, USA). every ~8000 bases, there is a lesion that prevents polymerase … Access Document

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117 purification kit (Axygen Incorporation) and sequenced in ABI 3730 sequencer (Applied Bi osystems, 128 of each primer (10 m M), 4 ml original template, 1 ml Taq polymerase and ddH 2O 27 ml. Amplification … Get Doc

The Prognostic Value Of P53 Mutation In Pediatric Marrow …
Manufacturer’s instructions (AXYGEN Biosciences, CA, USA). p53 DNA amplification was performed by real- Taq polymerase 2.5 U (0.5μL), MgCl 2 100 mM (5 μL), H 2O (42.5 μL), and p53 DNA template (10μL). … Access Content

POTENTIAL PROBLEMS WITH THE RELIABILITY OF PCR BASED …
Filter tips (Axygen) were used for all steps,exceptwhenwaterpumpwasused.AutoclavedMilliQwater(18.2M )was used without DEPC treatment. Sequencing of PCR products PCRproductwasre-amplifiedusingTaqpolymerase(Invitrogen,USA)accord- … Fetch This Document

ISOLATION AND PHYLOGENETIC ANALYSIS OF MARINE FUNGUS …
AxyPrep DNA gel extraction kit by Axygen Biosciences, USA. Purity of DNA was checked again on gel. 2.5. Sequencing of the purified PCR product The sequencing reactions were carried out using Taq DNA polymerase dye terminator cycle … Fetch Doc

BACTERIAL AND ARCHAEAL DIVERSITY IN ABYSSAL COLD SEEP …
Concentration of 0.2 μM, 1.25 U of Taq DNA polymerase (Takara, Japan), and 0.5 to 1 μl of DNA purification kit (AXYGEN, USA), then ligated into the pGEM-T Easy Vector (Preomega, … Fetch Here

The Complete Mitochondrial Genome Of Parafronurus Youi …
Each primer, 5 μM; 1 µl DNA template; 0.5 µl Takara Taq (or Takara LA Taq) DNA polymerase (Takara Biomedical, Japan). products were puriļ¬ed using the Axygen agarose-out kit and sequenced using ABI 3730 system by primer walking. … Fetch Content

Centre For Applied Molecular Biology, University Of The …
35 T-300Tips cat # 301-03-051 (Axygen) 01 Pack 36 Synthesize Dye-labeled Primers (See Picture # 01) 37 Synthesis of Primers for HPLC Purified (See Picture # 02) 38 Scissors Medium 02 39 Scissors Large 02 40 Taq DNA Polymerase (EP0402) 10 … Fetch Here

Nucleotide-Sugar Transporters In Plants – Centre Of Latin …
Taq DNA polymerase 0.2 [94 0.30 57.9 1.00 72 1.30] *30 72 10 4∞ Extension time was Plasmids were then purified from the cultures using an Axygen Mini Prep … View Doc

Real-timequantitativeRT-PCRforlow-abundancetranscriptsin …
1.25 U Taq polymerase (GeneChoice) and 18.75 Wlof H 2O. The cycling parameters were: 95‡C for 1.5 min; low-residue tips (Axygen Scienti¢c, Union City, CA, USA). Each sample, loaded in quadruplicates, had a … Document Viewer