Applied Biosystems Taq Polymerase Protocol

TaqMan – Wikipedia, The Free Encyclopedia
Reported in 1991 by researchers at Cetus Corporation, and the technology was subsequently developed by Roche Molecular Diagnostics for diagnostic assays and by Applied Biosystems for research applications. The TaqMan probe principle relies on the 5´–3´ exonuclease activity of Taq polymerase to cleave … Read Article

Standard Operating Procedure – Emily Chen, John Quackenbush …
This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, (Applied Biosystems; Cat # N808-0156) -10X PCR Buffer II -MgCl 2 Solution -Platinum Taq Polymerase … Read Here

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United States Department Of Agriculture Center For Veterinary …
(Applied Biosystems, Part #: 4311806) Note: Although a different brand name of Taq polymerase can be used, it 4.1.3 From this point, follow the protocol that is provided in the Qiagen DNeasy … Doc Retrieval

Real-time polymerase Chain Reaction – Wikipedia, The Free …
Real-time PCR can also be applied to the detection and quantification of DNA in samples to determine the presence and abundance of a particular of the reporter to the quencher prevents detection of its fluorescence; breakdown of the probe by the 5' to 3' exonuclease activity of the Taq polymerase … Read Article

NovaTaqTM DNA Polymerase And Kits
Standard Taq Polymerase and can eliminate the presence of nonspecific amplification such as up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Ca t. No. User Protocol TB309 Rev. A 0204 Page 2 of 5P age … Retrieve Full Source

TaqMan Gene Expression Assays Protocol 4357806A
• A PCR Master Mix and DNA polymerase (purchased separately) Note: You can choose from one of two Applied Biosystems 12 TaqMan Gene Expression Assays Protocol Applied Biosystems documents Documents Part Number … Read Full Source

Selection Of Reagents For Real-Time PCR
The goal of this document is to assist researchers in selecting the appropriate Applied Biosystems Protocol P/N: 4322169 . Kit Description: The High-Capacity cDNA Archive Kit contains all Taq Gold ® DNA Polymerase (50 µl) 100 U AmpErase ® … Return Doc

Chapter 6 – David N. Keys, Janice K. Au-Young, And Richard A …
Tion chambers are preloaded with user selected or predefined panels of Applied Biosystems TaqMan Gene The exonuclease activity of Taq DNA polymerase is responsible for hydro described in this protocol, for every 175 cells present at the 4. … Read Full Source

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Taq DNA Polymerase-Taq Polymerase
PowerQ® Taq DNA Polymerase for qPCR User Manual Tel :+86-21-54460832 E-mail:master@shinegene.org.cn 3、Protocol using other instruments, e.g. from Applied Biosystems, Bio-Rad … Retrieve Doc

AmpliTaq
Applied Biosystems AmpliTaq DNA Polymerase is a recombinant, thermostable, 94 kDa DNA polymerase encoded by a modified form of the Thermus aquaticus DNA … Access Document

Taq DNA Polymerase, Recombinant
"Hot-start" Protocol In the "hot-start" method, the addition of Taq DNA Polymerase is withheld purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. … Retrieve Here

SNP Genotyping – Wikipedia, The Free Encyclopedia
Taq DNA polymerase’s 5’-nuclease activity is used in the TaqMan assay for SNP genotyping. SNPlex is a proprietary genotyping platform sold by Applied Biosystems. Sequencing … Read Article

Applied Biosystems Taq Polymerase Protocol Images

PowerQ® Taq DNA Polymerase
PowerQ® Taq DNA Polymerase for qPCR User Manual 93℃ 2min→93℃5 sec→60℃30 sec 40cyclers 2、Protocol using LightCycler with Molecular Beacon probe 93℃ 2min→93℃5 sec→60℃20 sec→72℃20 sec 40cyclers 3、Protocol using other instruments, e.g. from Applied Biosystems, Bio-Rad … Read Document