Ampli Taq Taq Polymerase

A Rapid Method For Sequencing Of RRNA Gene(s) Amplified By …
Such studies ribosomal RNA gene(s), are ampli- fied from the genome of any organism using the polymerase chain reaction (3), cloned and the purified genomic DNA (IO), 5 pI of 10 x Taq DNA polymerase buffer (500 mM KCI, 100 mM Tris- … Retrieve Here

Amplicon – Wikipedia, The Free Encyclopedia
An amplicon is a piece of DNA formed as the product of natural or artificial amplification events. For example, it can be formed via polymerase chain reactions (PCR) or ligase chain reactions (LCR), as well as by natural gene duplication. Artificial amplification can be used to determine sex … Read Article

Takara’s Real Time PCR/RT-PCR Products Perfect Real Time …
ampli cation Amplification efficiency and versatility: RR041 > RR081 RR041 is recommended for the following cases: This premix includes Takara’s high-performance Ex Taq™ hot Start DNA Polymerase, which uses antibody-mediated Hot Start … Read Here

Understanding Variability In DNA Amplification Reactions Page …
Taq ™ DNA Polymerase offers a robust solution to the vast majority of endpoint PCR needs and shows exceptional performance against other robust performance with more difficult ampli-cons, such as those with high GC or AT content . … Access Doc

EEIIIIIII Technical Tips
Quence with Taq polymerase but not with Pfu or Deep Vent polymerases. In this report we present a successful ampli- fication of a viral sequence with primers … Document Retrieval

PCR Amplification Introduces Errors Into Mononucleotide And …
polymerase Pfu in place of Taq restricted the occurrence of such errors only in the case of short monothymidine repeats. We think that contraction of the repeat itself is the most com- … Get Content Here

Organismal And ‘gametic’ Capture-recapture Using …
AmpliTaq or Taq Gold DNA polymerase (0.5u) and 1µl of working DNA extract (approximately 50 ng). Temperature profiles were one initial cycle of 3’ or 10’ (for Taq Gold) at 94°C followed by 35 cycles each of 30’’ at … Doc Viewer

PyroPhage 3173 DNA Polymerase
The enzyme initiates efficiently at nicks, allowing ampli-fication to be initiated either with primers or at enzymatically-introduced nicks. Polymerase can be used in essentially the same way as Taq DNA polymerase. … Read Document

Archaeal DUTPase Enhances PCR Amplifications With Archaeal …
Longer targets in higher yield than Taq DNA polymerase. In vivo, archaeal dUTPases may play an essential role in preventing dUTP A 10-kb target was ampli fied from phage DNA by using 2.5 units of Pfu DNA polymerase in the absence ( , … Read More

CLONTECH – 2 PCR Enzyme System User Manual
Taq polymerase is ~3 kb (and much lower in many applications), Advantage 2 gives consistent and efficient amplifications of up to 18 kb or more tions higher than 0.2 mM of each dNTP when ampli fying large templates. However, we have had no … Read Document

Clinical Utility Of Polymerase Chain Reaction For Diagnosis …
Polymerase chain reaction (PCR) is a molec-ular biology technique that can detect M. tuberculosis 0.5µM of each outer primer, and 0.5U of Taq poly-merase (Ampli Taq; Perkin Elmer, Cetus, Norwalk, CT, … Fetch Full Source

History Of polymerase Chain Reaction – Wikipedia, The Free …
This DQα test is developed by Cetus as one of their "Ampli-Type" kits, and became part of early protocols for the testing of forensic evidence On December 22, 1989 the journal Science awarded Taq Polymerase (and PCR) its first "Molecule of the Year". … Read Article